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Tomlinson, Philip Ford
Superoxide scavenging, hydrogen peroxide deactivation, and benzoapyrene chemoprotective activities of a Maharishi Ayurveda food supplement, Maharishi Amrit Kalash.

Order No.9427918

Maharishi Ayurveda, a recent restoration of the traditional health care system of India, upholds Maharishi Amrit Kalash (MAK)--an herbal fruit concentrate (MAK-4) and an herbal tablet (MAK-5)--as a rasayana, a food supplement which promotes physiological balance, health, and immunity. Antioxidant and anticarcinogenic activities of MAK have been previously demonstrated in biochemical, cell culture, and in vivo studies. In the present investigation, superoxide scavenging and hydrogen peroxide deactivation properties of MAK were quantified in enzymatic assays, and the ability of MAK to scavenge reactive oxygen species within HeLa cells and to protect C3H/10T1/2 mouse embryo fibroblast-like cells from benzo (a) pyrene transformation was determined.

The superoxide scavenging properties of MAK were investigated with superoxide radicals generated during the catalytic activity of xanthine oxidase. Solutions containing MAK-4 and MAK-S (7.5 and 2.0 mg dry weight of extract/ml) inhibited the reduction of nitroblue tetrazolium (NBT) 96% and 98%, respectively. NBT reduction was decreased 50% by 30 micro g dry weight of extract/ml MAK-4 or 94 micro g dry weight of extract/ml MAK-5. Ascorbic acid inhibition of superoxide radical reduction of NBT reached 88% at 0.176 mg/ml, but declined to 42% at a concentration of 1.76 mg/ml. The rate of uric acid production monitored at 290 nm demonstrated negligible inhibition of xanthine oxidase by MAK-4, MAK-5, or ascorbic acid.

The ability of MAK to deactivate hydrogen peroxide was measured by determining the extent to which MAK inhibited the reduction of scopoletin by H sub 2 O sub 2 generated during the catalytic activity of glucose oxidase. At what appear to be approximately physiological concentrations (0.75 and 0.20 mg dry weight of extract/ml), MAK-4 and MAK-5 inhibited loss of scopoletin fluorescence 79% and 98%, respectively.

In cell culture, extracts from 1 micro g/ml MAK-4 and 10 micro g/ml MAK-5 inhibited the transformation of C3H/10T1/2 cells by benzo(a) pyrene 54% and 56%, respectively. Extracts from 20 micro g/ml MAK-4 and 15 micro g/ml MAK-5 inhibited intracellular reactive oxygen species generated by HeLa cells and monitored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide reduction 12% and 17%, respectively.

The results contribute to an understanding of previously reported anticarcinogenic, antioxidant, and anti-aging properties of MAK-4 and MAK-5, and warrant consideration in the light of present preventive, nutritional, and chemotherapeutic approaches to health, antioxidant defense, and carcinogenesis. Source: DAI, 55, no. 06B, (1994): 2120

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